Noha Mohammed Afifi Amin
Cairo University, Egypt
Title: Myelo-enhancement by astragalus membranaceus in male albino rats with chemotherapy myelosuppression: Histological and immunohistochemical study
Biography
Biography: Noha Mohammed Afifi Amin
Abstract
Myelo-suppression is the most common toxicity encountered in the oncology clinic today. This study was planned to investigate the possible protective and therapeutic role of the traditional Chinese Medicinal Herb; Astragalus membranaceus (AM), on chemotherapy-induced myelo-suppression. This study was carried out on thirty six adult male albino rats. They were divided into: Group-I Control received phosphate buffered saline (PBS) solution. Group-II were injected IP with cyclophosphamide (CY) for 3 days (IIa) and continued for one more week to receive AM orally (gIIb). Group-III received CY IP together with AM orally for 3 days. Group-IV received AM orally for one week (gIVa) and continued for extra three days receiving CY IP with AM orally (gIVb). Blood samples were analyzed for Total Leucocytic Count and Lymphocytic Count. Counting of CD34 positive cells in bone marrow was performed by flowcytometry. Bone marrow sections were subjected to H&E stain as well as immunohistochemical staining for anti- CD20 antibody. The mean area percentage of cellular bone marrow regions occupied by developing hematopoietic cells, mean area of fat cells and mean number of CD20 immunopositive B-lymphocytes in the bone marrow were measured by histomorphometric studies and statistically compared. AM proved to have a myelo-protective and myelo-therapeutic capacity, evidenced at both laboratory and morphological levels. The greatest myelo-potentiating effect of AM was achieved when supplied before and together with CY therapy.